Restriction endonucleases induce chromosomal aberrations in barley

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Restriction endonucleases induce chromosomal aberrations in barley.

The clastogenic ability of the restriction endonucleases (MspI, HpaII and HaeIII) in germinating seeds of reconstructed barley karyotype was assessed. An effective induction of chromosomal aberrations after restrictase treatment was observed. The frequency, types and cell-cycle dependence of the observed abnormalities are discussed in relation to the distinct characteristics of the enzymes and ...

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The endonucleases from the Type IIB restriction-modification systems differ from all other restriction enzymes. The Type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder of the DNA on a short duplex. The fact that ...

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ژورنال

عنوان ژورنال: Mutagenesis

سال: 1996

ISSN: 0267-8357,1464-3804

DOI: 10.1093/mutage/11.1.119